An extraction-free multiplex RT-PCR assay for simultaneous detection of six respiratory pathogens using polychromatic melting curve analysis
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Objectives: Acute respiratory infections commonly result from a spectrum of pathogens, including SARS-CoV-2, influenza A (IAV) and B viruses (IBV), respiratory syncytial virus (RSV), human adenovirus (HAdV), and Mycoplasma pneumo... ...