Comparative Metabarcoding of ITS1, ITS2, and Full-Length ITS Reveals Marker- and Tissue-Specific Variation in Fungal Community Profiling in Potato
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Accurate profiling of plant-associated fungal and oomycete communities depends critically on the choice of ITS marker. Using Illumina sequencing for ITS1/ITS2 and PacBio HiFi sequencing for full-length ITS (ITSf), we compared read recovery, taxonomic assignmen... ...