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A novel 3R-compliant ex vivo approach to assess ectoparasiticide repellency against Phlebotomus perniciosus in dogs

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Ectoparasiticides are essential for preventing insect bites and transmission of arthropod-borne pathogens such as Leishmania spp. Traditionally, their anti-feeding efficacy is evaluated in vivo using sedated dogs exposed to sand flies. To comply with 3R princi... ...