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Investigation of Drug Responses in 3D Tumor Spheroid Models Using Two-Photon Scanning Structured Illumination Super-Resolution Microscopy with Frequency-Specific Denoising Enhancement

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Two-dimensional cell culture models have long been a cornerstone of biomedical research; however, they often fail to accurately replicate the in vivo environment. In recent years, three-dimensional (3D) cell cultures, particularly 3D spheroid models, have gain... ...