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Development of a bioluminescent enzyme immunoassay for glycocholic acid based on a single-chain variable fragment-nanoluciferase fusion

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As glycocholic acid (GCA) has been recognized as a biomarker for hepatocellular carcinoma (HCC), its detection is crucial for the prevention, diagnosis, and treatment of HCC. With the advantages of being simple, rapid and sensitive, immunoassay is one of the o... ...