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Chromatographic resolution and reliable quantification of eleven endogenous and microbiota-derived tryptophan metabolites in human plasma by HPLC-MS/MS

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Tryptophan metabolism plays a central role in host-microbiota interactions and immune regulation, yet the simultaneous quantification of metabolites from the kynurenine, serotonin, and indole pathways remains analytically challenging due to their diverse physi... ...