Flush With Data (or) Optimizing and Validating the Efficacy of Free and Computationally Simple 16S Metabarcoding Approaches for Use in Wastewater Surveillance
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We propose free and low-computationally complex methods of 16S rRNA metabarcoding analysis, then optimized and validate their accuracy for wastewater bacterial surveillance. Three taxonomic analysis pipelines were augmented: NCBI BLAST subsampling, Kraken 2/Br... ...
