Genotype-specific optimization of in vitro regeneration and Agrobacterium-mediated transformation in indica rice with 35S:RUBY and CRISPR/LbCas12a system
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This study establishes a genotype-specific transformation system for indica rice cultivars Ranjit, Mahsuri, and Kon Joha using 35S:RUBY and CRISPR/LbCas12a constructs, enabling functional genomics studies and genetic improvement. The indica rice subspecies gen... ...
