Poly-thymine-mediated fluorescence recovery of N-CQDs for turn-on sensing of APE1 activity in breast cancer cell lysates
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A label-free turn-on fluorescent biosensor is developed for APE1 activity detection based on APE1-gated release of poly-T strands from a constrained DNA duplex, which competitively displaces Ag+ from N-CQDs, recovering fluorescence. The sensor achieves a detec... ...