Optimization of Erythrocyte Lysis Protocols for Robust and Reproducible T Cell Analysis in Murine Peripheral Blood, Spleen, Lung, and Bone Marrow
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Flow cytometry analysis of T lymphocytes in murine peripheral blood, spleen, lung, and bone marrow is critical for immune studies. However, inconsistent erythrocyte lysis remains a major challenge-incomplete lysis obscures lymphocyte detection, while excessive... ...
