Immunoglobulin depletion-based affinity purification of human serum haptoglobin without mannosylated IgM co-elution
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Haptoglobin is a major plasma glycoprotein and is widely utilized as a clinically relevant biomarker. However, we found that conventional single-step immunoaffinity purification using mono- or polyclonal anti-haptoglobin antibodies consistently resulted in the... ...