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Programmable spatiotemporal control of CRISPR-Cas12a: Engineering precision for next-generation gene editing and diagnostics

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CRISPR-Cas12a has emerged as a versatile alternative to Cas9, offering distinct advantages, such as recognition of thymine-rich protospacer adjacent motifs, generation of staggered 5' DNA breaks, simplified guide RNA architecture, and collateral (trans) singl... ...