Quantitative and Visual Detection of Pseudouridine at Specific Sites Using Bisulfite-Assisted Rolling Circle Amplification
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Pseudouridine (Ψ) is a prevalent RNA modification whose site-resolved quantification is critical for understanding epitranscriptomic regulation. We present pseU-SCOPE, a ligation-assisted rolling circle amplification (RCA) assay that converts a bisulfite-deri... ...
