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Comparative evaluation of noncanonical amino acids as site-specific NMR probes for the complex of E. coli SSB with single-stranded DNA without isotope labelling

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The binding of tetrameric E. coli single-stranded DNA-binding protein (SSB) to single-stranded DNA (ssDNA) was investigated using genetically encoded noncanonical amino acids (ncAA) as site-specific probes for detection by nuclear magnetic resonance (NMR) spec... ...