Overproduction of a recombinant heat-stable catalase for use in an extremophile enzyme lab exercise
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The manganese-dependent catalase of Pyrobaculum calidifontis, a facultative aerobe that grows optimally at 90 ˚C, has been previously used in a teaching lab to highlight salient characteristics of hyperthermophilic extremophiles. However, accruing enough enzy... ...
