Reversible Chemogenetic Fluorescence Labeling with pFAST in C. elegans
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The promiscuous fluorescence-activating and absorption-shifting tag (pFAST) enables reversible chemogenetic labeling with multiple fluorogens. We generated a single-copy tandem pFAST (td-pFAST) transgenic Caenorhabditis elegans strain expressed in pharyngeal m... ...