Comparative Evaluation of Duplex and Singleplex qPCR Assays for Detection of Human-Associated HF183 and General Bacteroides Markers and Implications for Duplexing in Digital PCR
{{output}}
Microbial source tracking (MST) using Bacteroidales markers provides information on faecal contamination in environmental waters. The marker BacUni targets a range of sources, while HF183 is primarily associated with human faeces. Duplex quantitative PCR (qPCR... ...