γ effects identify preferentially populated rotamers of CH2F groups: side-chain conformations of fluorinated valine analogues in a protein
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Using cell-free protein synthesis, the protein G B1 domain (GB1) was prepared with uniform high-level substitution of valine by (2 S ,3 S )-4-fluorovaline, (2 S ,3 R )-4-fluorovaline or 4,4'-difluorovaline. The 19 F nuclear magnetic resonance (NMR) signals... ...
