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Detection of Cardiac O-GlcNAcylation via Subcellular Fractionation and Dual Antibody Analysis in Pressure Overload Cardiac Hypertrophy

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Protein O-GlcNAcylation is a dynamic post-translational modification with emerging roles in cardiac pathophysiology. The availability of different pan-specific antibodies to assess global O-GlcNAc levels, and variability in western blot results has hindered cr... ...