Development of a real-time loop-mediated isothermal amplification (real-time LAMP) assay for the onsite detection of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (AHPND) in shrimp
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Acute hepatopancreatic necrosis disease (AHPN) is a lethal shrimp disease caused by Vibrio parahaemolyticus carrying virulent pirAB genes in extrachromosomal plasmids. However, the limitations of current polymerase chain reaction (PCR) methods pose challenges ... ...