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Expression, purification, and in vitro characterization of the carboxylesterase CEST-9.2 from Caenorhabditis elegans

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The nematode Caenorhabditis elegans biosynthesizes the ascarosides, a large, modular family of pheromones that are used in chemical communication. A number of carboxylesterase-like (CEST) enzymes are responsible for decorating the glycolipid core of the ascaro... ...