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Understanding the catalytic mechanism of the triazine hydrolase from Arthrobacter aurescens TC1

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The catalytic mechanism of the Zn(II)-dependent triazine hydrolase (TrzN) from Arthrobacter aurescens TC1 was examined by measuring the pH dependence of the Michaelis constants kcat, Km, and kcat/Km, the solvent isotope effect, and the thermodynamic parameters... ...