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Methanol response of V171S lipase from Geobacillus sp. 42 explored via experimental and in silico approaches

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The stability of lipase in organic solvents is crucial for biocatalytic processes in industrial biotechnology. Previously, we described a thermostable and solvent-tolerant Lip 42 from Geobacillus sp. Despite these features, the wild-type Lip 42 activity deteri... ...