An optimized click chemistry method allows visualization of proliferating neuronal progenitors in the mouse brain
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We establish a click reaction-based workflow (tissue clearing coupled with click-chemistry in 3D, C4-3D) to visualize 5-ethynyl-2'-deoxyuridine (EdU) in whole mouse brain tissue cleared by CUBIC, iDisco+, and PACT. C4-3D was compatible with immunostaining, nu... ...
