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Scalable extracellular expression of tag-free MPT64 protein in E. coli via pelB signal optimization: a step toward tuberculosis diagnostic antigen preparation

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Tuberculosis (TB) remains a global health burden, requiring affordable and scalable diagnostic tools. The MPT64 protein is a secreted biomarker specific to Mycobacterium tuberculosis and holds promise for rapid antigen-based diagnostics. However, existing reco... ...