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Real-Time Monitoring of the Dynamics and Heterogeneity for Mitochondria and Nucleoli with a Superphotostable Fluorescent Probe by Fluorescence Lifetime Microscopy

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Real-time monitoring of the dynamics and heterogeneity of mitochondria and nucleoli is essential for a comprehensive understanding of cellular homeostasis and pathological mechanisms. However, current approaches mainly suffer from two major limitations: (1) Li... ...