Designing Novel Aspartic Protease (NAP) Using Ancestral Sequence Reconstruction (ASR) for Cheesemaking
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Rhizomucor miehei protease (RMP), a chymosin-like fungal enzyme, is among the most widely used non-animal milk coagulant enzymes (MCEs) in cheese production. However, like other current MCEs, RMP exhibits non-specific proteolytic activity and high thermostabil... ...