LC-MS/MS-based determination of 25-hydroxyvitamin D in dried blood spots: crucial importance of combining a robust extraction with knowledge of the hematocrit
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Background: While many liquid chromatography - tandem mass spectrometry (LC-MS/MS)-based methods exist for the determination of 25-hydroxyvitamin D (25OHD) using dried blood spots (DBS), none of these comprehensively evaluated th... ...