[Substrate Behavior of Dissimilar Cy5-Deoxypyrimidine Nucleotides in PCR with DNA Templates of Different GC Compositions]
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The substrate properties of six pairs of fluorescently labeled deoxyuridine and deoxycytidine triphosphates (Cy5-dUTPs and Cy5-dCTPs) in PCR with Taq polymerase were compared. In each pair, the modified dU and dC contained identical fluorescently labeled Cy5 s... ...
