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3 Biotech. 2025 Jul;15(7):211. doi: 10.1007/s13205-025-04385-4 Q22.92025

Production of ethyl butyrate by a triple cascade process of the continuous submerged and solid state fermentation with corn stover

基于玉米秸秆的连续液态和固态发酵三级联产丁酸乙酯的过程 翻译改进

Dongsheng Xue  1, Jie Huang  1, Jueyan Chen  1, Qinyi He  2  3, Xuhao Zeng  2  3, Xiaowei Chen  1, Zhiyong Chen  1, Dejun Duan  1, Xihuo You  2  3

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作者单位

  • 1 National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, 430068 People's Republic of China.
  • 2 School of Bioengineering and Health, State Key Laboratory of New Textile Materials and Advanced Processing Technologies, Wuhan Textile University, Wuhan, 430200 People's Republic of China.
  • 3 Agrichina Huawei Biopharmaceutical (Hubei) Co, Ltd, Qichun 435300 People's Republic of China.
  • DOI: 10.1007/s13205-025-04385-4 PMID: 40521218

    摘要 中英对照阅读

    The gas lift bioreactor, the anaerobic fermentor and the anaerobic solid state fermentor were connected to continuously produce ethyl butyrate with corn stover as the substrate. The activities of the cellulase from the immobilized Aspergillus niger and the esterase from the immobilized Saccharomyces cerevisiae were 9.16 U/mL and 29.16 U/mL in the gas lift bioreactor. The hydrolysis ratio of the corn stover celluose was 55.4% in the solid-state fermentor. D... ...点击完成人机验证后继续浏览

    将气体提升生物反应器、厌氧发酵罐和固态厌氧发酵罐连接起来,以玉米秸秆为底物连续生产丁酸乙酯。固定化Aspergillus niger的纤维素酶活性和固定化Saccharomyces cerevisiae的酯酶活性分别为9.16 U/mL 和29.16 U/mL,在气体提升生物反应器中,玉米秸秆纤维素的水解率为55.4%。从连续发酵的第40.0小时到第120.0小时,丁酸乙酯浓度为30.55 g/kg。总丁酸与总醋酸的比例以及总丁酸与释放葡萄糖的比例分别为6.72和0.55。在不添加外源纤维素酶和酯酶的情况下,预处理后的玉米秸秆能够在好氧液态发酵和厌氧固态发酵的生物反应器级联中高效水解并连续生产丁酸乙酯。亮点: - 通过好氧与厌氧发酵串联过程生产丁酸乙酯。 - 实现了玉米秸秆的原位糖化以及乙醇和丁酸的原位酯化。 - 使用玉米秸秆为底物连续生产丁酸乙酯。 - 将纤维素转化为丁酸乙酯的代谢负担由三种微生物协同承担。

    关键词:生物反应器级联;丁酸;连续发酵;玉米秸秆;丁酸乙酯。

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    期刊名:3 biotech

    缩写:3 BIOTECH

    ISSN:2190-572X

    e-ISSN:2190-5738

    IF/分区:2.9/Q2

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    Production of ethyl butyrate by a triple cascade process of the continuous submerged and solid state fermentation with corn stover