3 Biotech. 2025 Jul;15(7):207. doi: 10.1007/s13205-025-04369-4 Q22.92025
Whole genome sequence analysis of a novel Streptomyces sp. BG6AG reveals its lipase production potential: optimization of the process through Taguchi experimental design
整个基因组序列分析揭示了新型链霉菌BG6AG的脂酶产生潜力：通过Taguchi实验设计优化生产过程 翻译改进
Zahra Parwez 1, Subhransu Sekhar Behera 1, Ananta Narayan Panda 1, Sudhansu Kumar Gouda 1, Lopamudra Ray 1
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DOI: 10.1007/s13205-025-04369-4 PMID: 40521213
摘要 中英对照阅读
本研究报道了一株从印度奥里萨邦奇利卡湖分离的产脂肪酶链霉菌BG6AG。全基因组分析显示其基因组大小为7,565,774碱基对，GC含量为72.2%。基因组分析利用KEGG、COG、antiSMASH、TYGS、CGView、RGI和RAST等生物信息学工具鉴定出6740个编码序列、59个tRNA分子和3个rRNA分子。生化测试确认了该微生物中存在脂肪酶。采用Taguchi统计设计优化了脂肪酶的生产，这是一种能够提高实验效率的稳健方法。结果表明，用于脂酶生产的最佳培养基是补充有丁酸三甘油脂、阿拉伯糖、天冬酰胺和Mn2+的最小盐培养基（MSM），pH值调整为9。培养基优化使脂肪酶产量提高了一倍。本研究结果有助于了解链霉菌BG6AG的基因组特征及其作为工业相关酶来源的潜力。
补充信息： 在线版本包含在10.1007/s13205-025-04369-4处可获得的补充材料。
关键词： 奇利卡湖；脂肪酶；链霉菌；Taguchi统计设计；全基因组分析。
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