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Annali dell'Istituto superiore di sanita. 2025 Apr-Jun;61(2):149-156. doi: 10.4415/ANN_25_02_08 Q31.72025

A preliminary study of the effects of transformations induced by gamma-ray treatment on the detection of Acheta domesticus and Tenebrio molitor allergenic proteins by enzyme-linked immunosorbent assay (ELISA) techniques

γ射线照射对革鲁布和粉斑螟过敏原检测影响的初步研究 翻译改进

Augusto Alberto Pastorelli  1, Matteo Rosetti  1, Silvia Di Giacomo  1, Emanuela Bortolin  2, Stefania Morelli  1, Antonella Semeraro  1, Concetta Boniglia  1, Paolo Stacchini  1, Raffaella Gargiulo  1

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作者单位

  • 1 Dipartimento di Sicurezza Alimentare, Nutrizione e Sanità Pubblica Veterinaria, Istituto Superiore di Sanità, Rome, Italy.
  • 2 Servizio Grandi Strumentazioni e Core Facilities, Istituto Superiore di Sanità, Rome, Italy.
  • DOI: 10.4415/ANN_25_02_08 PMID: 40519019

    摘要 中英对照阅读

    Introduction: With the global population projected to reach approximately 9 billion by 2050, there's a growing need to explore alternative food sources. Insects have emerged as a potential solution to meet food demand, offering a substitute for conventional livestock. However, a primary safety concern surrounding these novel foods is their allergenic potential, especially given the absence of standardized testing methodologies. To mitigate this risk, food irradiation has been explored as a method to reduce allergenicity in insects intended for human and animal consumption.

    Material and methods: This study utilized an enzyme-linked immunosorbent assay to determine the allergenic proteins in specific insect types after irradiation treatment.

    Results and discussion: Significant differences in detectable protein levels were observed between Tenebrio molitor and Acheta domesticus samples, but no significant differences in protein content were found between food and feed samples of both species under identical irradiation conditions. Further research is required to ensure the protocol's suitability for more complex food matrices.

    Keywords:gamma-ray treatment; allergenic proteins

    简介: 随着全球人口预计到2050年将达到约90亿,探索替代食品来源的需求日益增长。昆虫作为满足食物需求的潜在解决方案已崭露头角,为传统家畜提供了替代选择。然而,围绕这些新型食品的主要安全问题之一是其过敏原性,特别是由于缺乏标准化测试方法。为了降低这一风险,人们探讨了使用食品辐照来减少供人类和动物食用昆虫的过敏原性。

    材料与方法: 本研究采用酶联免疫吸附试验测定在辐照处理后特定种类昆虫中的过敏原蛋白。

    结果与讨论: 在相同辐照条件下,黄粉虫(Tenebrio molitor)和家蟋蟀(Acheta domesticus)样本之间可检测到的蛋白质水平存在显著差异。然而,在这两种物种的食物和饲料样本中未发现蛋白质含量有显著变化。需要进一步研究以确保该协议适用于更复杂的食品基质。

    关键词:伽马射线治疗; 过敏原蛋白; 酶联免疫吸附测定

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    期刊名:Annali dell istituto superiore di sanita

    缩写:ANN I SUPER SANITA

    ISSN:0021-2571

    e-ISSN:2384-8553

    IF/分区:1.7/Q3

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    A preliminary study of the effects of transformations induced by gamma-ray treatment on the detection of Acheta domesticus and Tenebrio molitor allergenic proteins by enzyme-linked immunosorbent assay (ELISA) techniques