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Journal of the mechanical behavior of biomedical materials. 2025 Jun 4:170:107094. doi: 10.1016/j.jmbbm.2025.107094 Q23.52025

Investigating simultaneous mineralization across layers during tooth development using atomic force microscopy and Raman spectroscopy

基于原子力显微镜和拉曼光谱的牙齿发育过程中牙本质分层矿化研究 翻译改进

Hutomo Tanoto  1, Hanwen Fan  1, Jacob Zachary Chen  2, Carla Berrospe Rodriguez  1, Ethan Milton  3, Fernanda Espinoza  1, Guillermo Aguilar  4, Connor P Dolan  5, Noriaki Ono  6, Yuxiao Zhou  7

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作者单位

  • 1 J. Mike Walker '66 Department of Mechanical Engineering, Texas A&M University, 3123 TAMU, College Station, TX, 77843, USA.
  • 2 Walker Department of Mechanical Engineering, The University of Texas at Austin, 204 E. Dean Keeton Street, Austin, TX, 78712, USA.
  • 3 Department of Mechanical Engineering, Vanderbilt University, 2301 Vanderbilt Place, Nashville, TX, 37240, USA.
  • 4 J. Mike Walker '66 Department of Mechanical Engineering, Texas A&M University, 3123 TAMU, College Station, TX, 77843, USA; Department of Materials Science and Engineering, Texas A&M University, 3003 TAMU, College Station, TX, 77843, USA.
  • 5 School of Life Sciences, Arizona State University, 550 E Orange Street, Tempe, AZ, 85281, USA.
  • 6 School of Dentistry, The University of Texas Health Science Center at Houston, 7500 Cambridge St, Houston, TX, 77054, USA.
  • 7 J. Mike Walker '66 Department of Mechanical Engineering, Texas A&M University, 3123 TAMU, College Station, TX, 77843, USA; School of Engineering Medicine, Texas A&M University, 1020 Holcombe Blvd, Houston, TX, 77030, USA. Electronic address: yzhou@tamu.edu.

    • DOI: 10.1016/j.jmbbm.2025.107094 PMID: 40513433

    摘要 中英对照阅读

    Tooth development is a complex multi-step biochemical process characterized by the sequential formation and maturation of dental tissues, with biomineralization playing a central role in the production of mineralized tissues essential for various biological functions. This study focuses on the later stages of tooth development, marked by intense biomineralization, during which enamel and dentin undergo crucial structural transformations necessary to fulfill the mechanical functions of the tooth. Atomic force microscopy (AFM) nanomechanical testing provided insights into the microstructures and mechanical properties of enamel and dentin during both the advanced bell stage and post-eruptive stage. Additionally, Raman spectroscopy measurements revealed variations in the biochemical properties from advanced bell stage to post-eruptive stage. AFM-based micro-rheology results demonstrated that the dental papilla extracellular matrix exhibits spatially heterogeneous viscoelastic responses to dynamic mechanical stimuli, suggesting potential region-specific roles in mechanotransduction during tooth development. These findings highlight the spatial heterogeneity of microstructural, mechanical and biochemical properties that emerge during the late stages of tooth formation.

    Keywords: AFM-Based micro-rheology; Atomic force microscopy (AFM) nanomechanical testing; Biomineralization; Raman spectroscopy; Tooth development.

    Keywords：tooth development; atomic force microscopy; Raman spectroscopy

    牙齿发育是一个复杂的多步骤生化过程，特征是牙组织的顺序形成和成熟，其中生物矿化在生产对各种生物学功能至关重要的矿化组织中发挥核心作用。本研究重点在于牙齿发育后期阶段，这一时期以强烈的生物矿化为标志，在此期间，釉质和 dentin（译者注：此处保留英文原文）经历关键的结构变化，以便满足牙齿的各种机械功能需求。原子力显微镜 (AFM) 纳米力学测试提供了关于牙本质在先进钟状期和萌出后阶段的微观结构和力学性能的见解。此外，拉曼光谱测量揭示了从先进钟状期到萌出后阶段生化性质的变化。基于 AFM 的微流变学结果表明，牙齿乳头细胞外基质对动态机械刺激表现出空间异质性的粘弹性响应，暗示其在牙齿发育过程中可能具有区域特异性的力学信号转导作用。这些发现突显了在牙齿晚期形成期间出现的微观结构、力学和生化性质的空间异质性。

    关键词：基于 AFM 的微流变学；原子力显微镜 (AFM) 纳米力学测试；生物矿化；拉曼光谱；牙齿发育。

    关键词：牙齿发育; 原子力显微镜; 拉曼光谱学

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    期刊名：Journal of the mechanical behavior of biomedical materials

    缩写：J MECH BEHAV BIOMED

    ISSN：1751-6161

    e-ISSN：1878-0180

    IF/分区：3.5/Q2

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