Ectoine is a substance produced by extremophiles and is naturally used by them as protection against adverse environmental conditions in which they live. Scientific contributions discuss its excellent effect through cosmotropic properties, prevention of secondary messenger release in cells, and transcription factors. The influence on the lipid layer of the cell membrane and its preventive effect as a UV filter were also demonstrated. What is more, its anti-oxidative effect was established. Ectoine works as an immunostimulant and also has anti-inflammatory and anti-cancer properties. These attributes are dominating factors in the use of ectoine's properties in skin fractionation treatment with a CO2 laser. In the following work, the influence of ectoine on skin parameters was described, focusing on redness, moisturization, and TEWL after the use of a CO2 laser (Załęska 2019). The rheological properties of preparations with ectoine addition were also tested. The yield point was determined, the viscosity changes of cosmetic preparations were measured with increasing shear rates, and oscillation tests were performed. With increasing percentages of ectoine and frequency of application, the occurrence of redness after CO2 therapy decreased. The highest moisture level values from 54.4 × 0.02 mg/cm2 to 72.5 × 0.02 mg/cm2 were obtained for preparation A applied twice a day; for the same preparation, a reduction in TEWL from 6.2 to 5.3 g/(m2·h) was obtained. The results of the tests of cosmetic emulsions allowed us to conclude that the preparations in the analyzed shear rate range at all tested temperatures are non-Newtonian liquids that are shear-thinning and have a flow limit. The obtained results of the conducted research prove the positive effect of dermocosmetics with ectoine content in the process of skin healing.

Keywords: ectoine; laser; skin; skin treatment.

ECToine是一种由极端嗜好者产生的物质，它们自然利用这种物质作为保护自己免受生活环境中的不利环境条件影响的手段。科学研究讨论了其通过亲水性作用、预防细胞中第二信使释放以及转录因子的作用所产生的卓越效果。此外还证明了它对细胞膜脂质层的影响及其作为紫外线过滤器的预防效果，并且确立了它的抗氧化效应。ECToine用作免疫刺激剂，也具有抗炎和抗癌特性。这些属性是使用ECToine在皮肤激光换肤治疗（如二氧化碳激光）中特性的主导因素。以下的工作描述了ECToine对皮肤参数的影响，重点关注在使用二氧化碳激光后的红斑、保湿以及TEWL（经皮水分流失）。还测试了添加ECToine的制剂的流变性质，确定了屈服点，并测量了化妆品制剂随剪切速率增加而变化的粘度，进行了振荡试验。随着ECToine百分比和应用频率的增加，在二氧化碳治疗后红斑的发生率降低。对于每天两次使用的产品A，获得的最大保湿水平值为54.4 × 0.02 mg/cm² 至 72.5 × 0.02 mg/cm²；同样地，该产品使TEWL从6.2降至5.3 g/(m²·h)。化妆品乳液的测试结果表明，在分析的所有剪切速率范围内和所有测试温度下，这些制剂是非牛顿液体，并且是剪切稀化的并且具有流动限制。所进行的研究获得的结果证明了含ECToine的皮肤护理产品在促进皮肤愈合过程中的积极效果。

关键词： ECToine；激光；皮肤；皮肤治疗。