Purpose: To evaluate the diagnostic accuracy and detection rate of lymph node metastases (LNM) in Papillary Thyroid Carcinoma (PTC) using the One-Step Nucleic Acid Amplification (OSNA) technique compared to conventional pathological methods.

Methods: A systematic search was conducted in PubMed, The Cochrane Library, Scopus, and Web of Science from May 1, 2023, to June 30, 2023, for studies published from January 1, 2005, to April 30, 2023. Observational studies assessing OSNA for LNM in PTC patients were included. Two independent reviewers performed study selection, record screening, data extraction, and quality assessment using QUADAS-2. Meta-analysis was conducted with Posit software (RStudio).

Results: Seven studies (2014-2021) involving 1,424 lymph nodes from 207 PTC patients were analyzed. The pooled sensitivity, specificity, and AUC of OSNA for detecting LNM were 0.905 (95% CI 0.838-0.946), 0.884 (95% CI 0.834-0.921), and 0.848, respectively.

Discussion: OSNA shows promising diagnostic accuracy compared to conventional pathological methods, with the potential to improve real-time lymphatic staging and aid intraoperative decision-making. Limitations include study heterogeneity and a lack of randomized controlled trials, affecting generalizability. Further research is needed to validate the long-term benefits of OSNA in clinical practice.

Keywords: Carcinoma, papillary; Lymphatic metastasis; Meta-analysis; Nucleic acid amplification techniques; Systematic review; Thyroid neoplasms.

© 2025. The Author(s).

目的：评估在乳头状甲状腺癌（PTC）中使用一步核酸扩增技术（OSNA）检测淋巴结转移（LNM）的诊断准确性及检出率，与传统病理方法进行比较。

方法：系统检索了PubMed、The Cochrane Library、Scopus和Web of Science数据库，时间范围为2023年5月1日至2023年6月30日，纳入的研究发表时间为2005年1月1日至2023年4月30日。包含评估OSNA在PTC患者中检测LNM的观察性研究。两名独立评审员使用QUADAS-2进行文献筛选、数据提取和质量评价。Meta分析使用Posit软件（RStudio）完成。

结果：共纳入七项研究（发表于2014年至2021年），涉及207名PTC患者的1,424个淋巴结。OSNA检测LNM的汇总灵敏度、特异性和AUC分别为0.905 (95% CI 0.838-0.946)、0.884 (95% CI 0.834-0.921) 和0.848。

讨论：OSNA显示出比传统病理方法更高的诊断准确性，有望改善实时淋巴结分期和术中决策。但研究异质性和缺乏随机对照试验限制了其普遍适用性，需要进一步的研究来验证OSNA在临床实践中的长期效益。

关键词：乳头状癌；淋巴转移；Meta分析；核酸扩增技术；系统综述；甲状腺肿瘤。

© 2025. The Author(s).