Objective: T2DM (type 2 diabetes mellitus) is a chronic metabolic disease that seriously affects human health. Abnormal expression of microRNAs has been reported to play an important role in disease diagnosis. This study aimed to investigate the predictive value of miR-4454 for T2DM and possible risk factors for comorbidity and complications (CC) of T2DM.

Methods: Baseline information was collected from a total of 206 subjects including 109 T2DM patients (T2DM group) and 97 healthy individuals (healthy control group), and miR-4454 expression levels were assessed by qRT-PCR. A chi-squared test and a t-test were used to assess the difference in miR-4454 expression of T2DM and healthy subjects. The predictive value of miR-4454 on T2DM was evaluated by receiver operating characteristic (ROC). Potential risk factors for CC of T2DM were predicted by multivariate logistic regression analysis.

Results: Significant downregulation of miR-4454 was observed in the T2DM group. The predictive value of miR-4454 for T2DM was verified by the ROC curve. MiR-4454 expression in the T2DM group was negatively correlated with fasting blood glucose (FBG), glycated hemoglobin (HbA1c), homeostasis model-insulin resistance index (HOMA-IR), total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), and positively correlated with the levels of adiponectin (ADI) and peroxisome proliferator-activated receptor gamma (PPAR-γ). Multivariate logistic regression analysis suggested that miR-4454, FBG, HbA1c, HOMA-IR, TC, TG, and LDL-C could be considered risk factors for CC of T2DM.

Conclusions: The expression level of miR-4454 showed a diagnostic value for T2DM and could serve as a risk factor for the occurrence of CC in T2DM.

Clinical trial number: Not applicable.

Keywords: Adverse outcome; Diagnostic value; T2DM; miR-4454.

© 2025. The Author(s).

目标: 二型糖尿病（T2DM）是一种严重影响人类健康的慢性代谢疾病。异常表达的microRNAs在疾病诊断中被认为扮演着重要角色。本研究旨在探究miR-4454对T2DM的预测价值及其可能影响共病和并发症（CC）的风险因素。

方法: 从总共206名参与者中收集基线信息，其中包括109名T2DM患者（T2DM组）和97名健康个体（健康对照组），并通过qRT-PCR评估miR-4454的表达水平。通过卡方检验和t检验来比较T2DM与健康受试者之间miR-4454表达量的差异。利用接收者操作特性曲线（ROC）评估miR-4454对T2DM的预测价值，并通过多元逻辑回归分析预测可能导致T2DM共病和并发症的风险因素。

结果: 在T2DM组中观察到miR-4454显著下调。通过ROC曲线验证了miR-4454对T2DM的预测价值。T2DM组中的miR-4454表达与空腹血糖（FBG）、糖化血红蛋白（HbA1c）、胰岛素抵抗指数（HOMA-IR）、总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）负相关，而与脂肪细胞因子（ADI）和过氧化物酶体增殖激活受体γ（PPAR-γ）的水平正相关。多元逻辑回归分析表明，miR-4454、FBG、HbA1c、HOMA-IR、TC、TG和LDL-C可以被认为是T2DM共病和并发症的风险因素。

结论: miR-4454的表达水平具有诊断T2DM的价值，并可作为T2DM发生共病和并发症的风险因素。

临床试验编号: 不适用。

关键词: 不良结局；诊断价值；T2DM；miR-4454.