Current microbiology. 2025 Jun 9;82(7):330. doi: 10.1007/s00284-025-04298-8 Q32.62025
Isolation, Screening, Identification of Cellulolytic Bacteria and Optimization of Factors Affecting FPUase Synthesis for Bagasse Hydrolysis
木质纤维素分解菌的筛选鉴定及FPU酶产酶条件优化研究 翻译改进
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DOI: 10.1007/s00284-025-04298-8 PMID: 40488897
摘要 中英对照阅读
筛选能够合成FPU酶的细菌以及优化碱性预处理蔗渣水解条件是当前研究的重点。本研究系统地从土壤、淤泥和牛肝样本中筛选出具有降解纤维素能力的细菌,并确定HL04为一种强大的FPU酶生产菌株。通过16S rRNA核糖体序列分析,确认HL04属于新发现的一种微生物——安全沙芽孢杆菌(Bacillus safensis),该微生物能够合成FPU酶。研究通过单因素实验优化了发酵时间、底物浓度、pH值和温度等条件下的FPU酶生产情况。采用响应面法(RSM)中的Box-Behnken设计(BBD),进一步精炼这些因子以提高FPU酶产量。所得二次多项式模型显示出了高度有效性(R2=0.9913),并且统计学上显著的交互作用(P
© 2025. 作者保留独家授权给Springer Science+Business Media, LLC(隶属于Springer Nature)的权利。
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