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Current microbiology. 2025 Jun 9;82(7):330. doi: 10.1007/s00284-025-04298-8 Q32.62025

Isolation, Screening, Identification of Cellulolytic Bacteria and Optimization of Factors Affecting FPUase Synthesis for Bagasse Hydrolysis

木质纤维素分解菌的筛选鉴定及FPU酶产酶条件优化研究 翻译改进

Le Duy Khuong  1, Van Minh Nguyen  2, Roan Thi Do  3, Huong Thi Thanh Doan  3, Don Nguyen  4, Sang Van Vu  5, Hong Thi Nguyen  6  7, Ichiro Kamei  8, Chu Luong Tri  9

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作者单位

  • 1 VNU School of Interdisciplinary Sciences and Arts, Vietnam National University, Hanoi, Vietnam. leduykhuong@vnu.edu.vn.
  • 2 VNU School of Interdisciplinary Sciences and Arts, Vietnam National University, Hanoi, Vietnam.
  • 3 Department of Immunology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
  • 4 Squalicum High School, Bellingham, WA, USA.
  • 5 Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.
  • 6 Environment and Sustainable Development Research Group, Dong Nai Technology University, Bien Hoa, Vietnam.
  • 7 Faculty of Technology, Dong Nai Technology University, Bien Hoa, Vietnam.
  • 8 Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen-kibanadai-nishi, Miyazaki, 889-2192, Japan.
  • 9 Faculty of Environment, Ha Long University, Uong Bi City, Quang Ninh, Vietnam.
  • DOI: 10.1007/s00284-025-04298-8 PMID: 40488897

    摘要 中英对照阅读

    The screening of FPUase-synthesizing bacteria and optimization of hydrolysis conditions for alkaline pre-treated bagasse are key focuses of current research. This study systematically screened cellulose‑degrading bacteria from soil, mud, and cow liver samples, identifying HL04 as a robust FPUase‑producing strain. Based on 16S rRNA ribosome sequence, HL04 was identified as Bacillus safensis, a newly discovered microorganism capable of synthesizing FPUase.... ...点击完成人机验证后继续浏览

    筛选能够合成FPU酶的细菌以及优化碱性预处理蔗渣水解条件是当前研究的重点。本研究系统地从土壤、淤泥和牛肝样本中筛选出具有降解纤维素能力的细菌,并确定HL04为一种强大的FPU酶生产菌株。通过16S rRNA核糖体序列分析,确认HL04属于新发现的一种微生物——安全沙芽孢杆菌(Bacillus safensis),该微生物能够合成FPU酶。研究通过单因素实验优化了发酵时间、底物浓度、pH值和温度等条件下的FPU酶生产情况。采用响应面法(RSM)中的Box-Behnken设计(BBD),进一步精炼这些因子以提高FPU酶产量。所得二次多项式模型显示出了高度有效性(R2=0.9913),并且统计学上显著的交互作用(P

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    期刊名:Current microbiology

    缩写:CURR MICROBIOL

    ISSN:0343-8651

    e-ISSN:1432-0991

    IF/分区:2.6/Q3

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    Isolation, Screening, Identification of Cellulolytic Bacteria and Optimization of Factors Affecting FPUase Synthesis for Bagasse Hydrolysis