This study focused on optimizing the enzymatic synthesis of biolubricants using a 20% blend of Nile tilapia (Oreochromis niloticus) and neem (Azadirachta indica) oils catalyzed by Eversa Transform 2.0. The optimal conditionsthe molar ratio of 1:5 (mol/mol), 10% biocatalyst load, and a reaction time of 48 hachieved a predicted ester conversion of 97%, with experimental validation confirming 92.80 ± 0.03%. Molecular docking demonstrated stable complexes, particularly with decanoic and linoleic acids, showing Root Mean Square Deviation (RMSD) values below 2.0 Å, indicating minimal conformational changes. Gas chromatography and mass spectrometry (GC-MS) analysis identified key esters like 2-ethylhexyl and octyl, which enhance thermal stability, lubricity, and oxidation resistance. Fourier Transform Infrared Spectroscopy (FT-IR) analysis confirmed effective ester formation, corroborating experimental results. Viscosity (2.4511 mm²/s) and density (0.84259 g/cm³) values aligned with ISO VG 3 (International Organization for Standardization Viscosity Grade) standards, suitable for low-load and low-speed applications.

© 2025 The Authors. Published by American Chemical Society.

本研究集中在使用尼罗罗非鱼（Oreochromis niloticus）和印度槐树（Azadirachta indica）油20%混合物，在Eversa Transform 2.0催化下，优化生物润滑剂的酶法合成。最优条件下——摩尔比为1:5 (mol/mol)，10%生物催化剂负载量以及反应时间为48小时——实现了预测酯转化率为97%，实验验证确认为92.80 ± 0.03%。分子对接展示了与癸酸和亚油酸的稳定复合体，显示了小于2.0 Å的均方根偏差（RMSD）值，表明构象变化极小。气相色谱-质谱联用分析（GC-MS）鉴定出关键酯类如乙基己基和辛基，这些酯可以提高热稳定性、润滑性和抗氧化性。傅里叶变换红外光谱（FT-IR）分析证实有效形成酯类，与实验结果一致。黏度值（2.4511 mm²/s）和密度值（0.84259 g/cm³）符合ISO VG 3（国际标准化组织粘度等级）标准，适用于低负载和低速应用。

© 2025 The Authors. Published by American Chemical Society.