Administration of high-dose fermented ginseng powder (2.385 mg/g) resulted in a reduction in body weight and an improvement in blood biochemical parameters in high-fat diet (HFD)-fed mice. Significant reductions in lipid droplet size were observed in both liver and epididymal adipose tissues. Western blot analysis showed increased protein levels of PPAR-α, PPAR-γ, and PGC-1 in the HFD + low-dose lyophilized fermented ginseng powder (HDL), HFD + medium-dose lyophilized fermented ginseng powder (HDM), and HFD + high-dose lyophilized fermented ginseng powder (HDH) groups compared to the HD group. Furthermore, the phosphorylation of AMPK (P-AMPK) and ACC (P-ACC) was significantly elevated. Conversely, western blot analysis demonstrated a decrease in the expression of inflammatory cytokines IL-1, IL-6, and TNF-α in the CG, HDL, HDM, and HDH groups compared to the HD group. Gene expression analysis revealed a downregulation of lipid anabolism-related genes, including SREBP-1c and FAS, along with an upregulation of PPAR-γ and ACOX-1 mRNA levels. Additionally, the expression of inflammation-related genes such as IL-1, IL-6, and TNF-α was reduced. High-dose freeze-dried fermented ginseng powder (2.385 mg/g) significantly influenced lipid metabolism and inflammatory responses, highlighting its potential as a therapeutic agent for the management of dyslipidemia.

Keywords: fermented ginseng; high-fat diet; lipid-lowering; modulating effect; obesity.

© 2025 Xueyue Tai et al.

高剂量发酵人参粉（2.385 mg/g）的管理使高脂饮食（HFD）喂养的小鼠体重减轻，并改善了血液生化参数。在肝脏和附睾脂肪组织中观察到脂滴大小显著减小。Western印迹分析显示，与HD组相比，HFD +低剂量冷冻干燥发酵人参粉（HDL）、HFD +中剂量冷冻干燥发酵人参粉（HDM）和HFD +高剂量冷冻干燥发酵人参粉（HDH）组的PPAR-α、PPAR-γ和PGC-1蛋白水平升高。此外，AMPK（P-AMPK）和ACC（P-ACC）的磷酸化显著增加。相反，Western印迹分析表明，与HD组相比，CG、HDL、HDM和HDH组中促炎细胞因子IL-1、IL-6和TNF-α的表达降低。基因表达分析显示，包括SREBP-1c 和 FAS 在内的脂质合成相关基因下调，同时观察到PPAR-γ 和 ACOX-1 mRNA水平上调。此外，炎症相关基因如IL-1、IL-6和TNF-α的表达降低。高剂量冷冻干燥发酵人参粉（2.385 mg/g）显著影响脂质代谢和炎性反应，显示出其作为治疗剂管理血脂异常的潜力。

关键词： 发酵人参；高脂饮食；降脂作用；调节效应；肥胖。

© 2025 太学月 等人。