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Response surface methodology for optimization of scFv-BAD anti-E2 CHIKV expression in Escherichia coli Origami B (DE3) for the detection of Chikungunya virus

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Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for recurring outbreaks, highlighting the need for rapid and accurate diagnostic tools. The E2 glycoprotein of CHIKV is a promising target for antibody-based detection. This study aims to deter... ...