Current microbiology. 2025 May 17;82(7):290. doi: 10.1007/s00284-025-04228-8 Q32.62025
Bioremediation Performance of Recombinant Shewanella azerbaijanica; Considering Uranium Removal in the Presence of Nitrate
自重组休瓦内拉·阿塞拜疆菌株考虑硝酸根存在下铀去除的生物修复性能 翻译改进
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DOI: 10.1007/s00284-025-04228-8 PMID: 40382480
摘要 中英对照阅读
微生物基因工程已成为从工业废水中去除污染物的一种有前景的方法。Shewanella azerbaijanica具有还原铀的能力。本研究考察了高硝酸盐浓度对原生菌株和重组菌株中铀生物还原的影响。细菌性能通过ICP-AES方法测量的铀生物还原程度以及在存在铀和硝酸盐的情况下无氧条件下的存活率(通过Neubauer计数室测定)进行了评估,时间间隔分别为24小时、1周、4周、4个月和9个月。尽管重组菌株的细胞数量低于野生型菌株,在含铀和硝酸盐条件下培养24小时后,其铀还原效率比野生型菌株高出20%。这表明基因改造增强了胞外电子转移(EET)。改进的生物修复效率可能归因于克隆的mtrC基因,该基因促进了Shewanella细菌中更有效的电子传递。此外,使用SDM方法失活napB基因可能会进一步增强铀的去除效果。这种高性能趋势在所有时间间隔内均保持一致。对于野生型S. azerbaijanica,在1周、4周、4个月和9个月后,铀去除率分别为74%、54%、96%和99%。而在重组细菌中,这些去除率分别增加到了91%、78%、96%和100%。通过X射线衍射(XRD)分析进一步证实了生物还原机制,验证了S. azerbaijanica在存在硝酸盐的情况下能够减少铀的能力。总体而言,这项研究确定重组细菌是未来金属生物还原研究的有前景候选者。
© 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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