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Journal of medical entomology. 2025 May 7:tjaf043. doi: 10.1093/jme/tjaf043 Q12.02025

Duplex PCR assay to determine sex and mating status of Ixodes scapularis (Acari: Ixodidae), vector of the Lyme disease pathogen

一种用于确定伯氏疏螺旋体病媒刺锥虫 Scalars 的性别和交配状态的双工 PCR 检测方法 翻译改进

Isobel Ronai  1  2, Julia C Frederick  3  4, Alec T Thompson  4  5, Prisha Sharma  3, Michael J Yabsley  4  5  6, Utpal Pal  7  8, Cassandra G Extavour  1  2  9, Travis C Glenn  3  4  10

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作者单位

  • 1 Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
  • 2 Howard Hughes Medical Institute, Chevy Chase, MD, USA.
  • 3 Department of Environmental Health Science, University of Georgia, Athens, GA, USA.
  • 4 Center for the Ecology of Infectious Diseases, Odum School of Ecology, University of Georgia, Athens, GA, USA.
  • 5 Southeastern Cooperative Wildlife Disease Study, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
  • 6 Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, USA.
  • 7 Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
  • 8 Virginia-Maryland College of Veterinary Medicine, College Park, MD, USA.
  • 9 Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
  • 10 Institute of Bioinformatics, University of Georgia, Athens, GA, USA.

    • DOI: 10.1093/jme/tjaf043 PMID: 40341388

    摘要 中英对照阅读

    Ticks are a major health threat to humans and other animals, through direct damage, toxicoses, and transmission of pathogens. An estimated half a million people are treated annually in the United States for Lyme disease, a disease caused by the bite of a black-legged tick (Ixodes scapularis Say, 1821) infected with the bacterial pathogen Borrelia burgdorferi. This tick species also transmits another 6 human-disease causing pathogens, for which vaccines are... ...点击完成人机验证后继续浏览

    蜱虫是人类和其他动物健康的重大威胁，通过直接损害、毒性反应以及传播病原体等方式造成危害。据估计，每年在美国有大约五十万人因莱姆病接受治疗，这是一种由感染了细菌病原体伯氏疏螺旋体（Borrelia burgdorferi）的黑脚蜱（Ixodes scapularis Say, 1821）叮咬引起的疾病。这种蜱虫还传播另外6种导致人类疾病的病原体，目前尚无针对这些病原体的有效疫苗。虽然I. scapularis成虫阶段具有性别二态性，但尚未确定可以作为性别特异性标记的雄性和雌性I. scapularis之间的DNA序列差异。在此，我们利用全基因组重测序和限制性位点相关DNA测序技术，鉴定出了I. scapularis（雄性异配生殖系统XY）中的性别特异性DNA序列。然后我们确定了一个雄性特异性标记，并将其作为分子性别识别方法（双链PCR）的基础来区分I. scapularis蜱虫的性别。此外，我们提供了证据表明这种分子性别鉴定方法可用于确定已与携带雄性决定精子的雄性交配并受精的成年雌性的交配状态。我们的分子工具允许对作为重要病原体传播媒介的I. scapularis进行交配和性别特异性生物学特征分析，这对于更好地了解其生物学特性以及控制蜱虫种群至关重要。

    关键词：RADseq；Y染色体；黑脚蜱；鹿蜱；空腹期；性别鉴定。

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    Copyright © Journal of medical entomology. 中文内容为AI机器翻译，仅供参考！

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    期刊名：Journal of medical entomology

    缩写：J MED ENTOMOL

    ISSN：0022-2585

    e-ISSN：1938-2928

    IF/分区：2.0/Q1

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    Duplex PCR assay to determine sex and mating status of Ixodes scapularis (Acari: Ixodidae), vector of the Lyme disease pathogen