The plant genome. 2025 Jun;18(2):e70035. doi: 10.1002/tpg2.70035 Q13.82025

Genome-wide association study and BSR-seq identify nitrate reductase-related genes in rice landraces (Oryza sativa L.)

基于全基因组关联分析和BSR-seq发掘水稻农家品种中的硝酸盐还原酶相关基因翻译改进

Shuangshuang Luo^{1 2}, Zuyu Liao², Shilv Huang³, Xiuzhong Xia², Zongqiong Zhang², Baoxuan Nong^{2 4}, Tongping Luo², Chenli Zhu², Can Chen², Hui Guo², Rui Feng², Yinghua Pan^{2 4}, Shuhui Liang², Yongcheng Li^{1 2}, Jianhui Liu^{1 2}, Yongfu Qiu^{1 4}, Danting Li^{2 4}, Xinghai Yang^{2 4}

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作者单位

¹ College of Agriculture, Guangxi University, Nanning, China.

² Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.

³ Microbiology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.

⁴ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning, China.

DOI: 10.1002/tpg2.70035 PMID: 40281663

摘要中英对照阅读

Nitrogen (N) is an essential nutrient for rice (Oryza sativa L.) growth and development. However, the lower nitrogen use efficiency (NUE) results in an N fertilizer surplus, which causes many environmental problems. In this study, genome-wide association studies were used to detect nitrate reductase (NR)-related loci in 419 rice landraces. Using the general linear model (GLM), mixed linear model (MLM), linear model (LM), and linear mixed model (LMM), we fo... ...点击完成人机验证后继续浏览

氮是水稻（Oryza sativa L.）生长和发育所必需的营养元素。然而，较低的氮利用效率（NUE）导致了过量施用氮肥，这引发了许多环境问题。在这项研究中，我们使用全基因组关联分析来检测419个水稻地方品种中的硝酸还原酶（NR）相关位点。通过一般线性模型（GLM）、混合线性模型（MLM）、线性模型（LM）和线性混合模型（LMM），我们在三个性状中分别发现了6、9、7和6个显著的单核苷酸多态性（SNP），这些SNP在p值小于1 × 10^-5时与之相关。此外，通过3 V多位点随机-SNP效应混合线性模型，在这三个性状中有98个SNP具有log odds比≥3的相关性。有趣的是，我们发现Chr1_15896481在GLM、MLM、LM和LMM模型中均显著相关。同时，这个显著位点与批量分离RNA测序的候选区域重叠。通过综合分析，我们在第1号染色体上确定了一个最可能的候选基因组区域，该区域位于15,627,420-16,084,761 bp之间。通过对这一区间内的基因进行功能注释、RNA测序和实时定量聚合酶链反应（RT-qPCR）分析，我们确定了五个可能影响NR活性的候选基因。Os01g0378400与OsNR1.2表现出相似的基因表达模式，并且属于参与植物氮代谢的NAC转录因子家族。Os01g0377700同源于一个铵转运蛋白基因（Cre06g293051）。Os01g0383700编码WD40结构域蛋白，Os01g0379400编码F-box蛋白，而Os01g0382800编码DYW型PPR结构域蛋白。这些发现为提高水稻育种中的NUE遗传改良提供了宝贵的基因资源。

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期刊名：Plant genome

缩写：PLANT GENOME-US

ISSN：N/A

e-ISSN：1940-3372

IF/分区：3.8/Q1

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Genome-wide association study and BSR-seq identify nitrate reductase-related genes in rice landraces (Oryza sativa L.)

基于全基因组关联分析和BSR-seq发掘水稻农家品种中的硝酸盐还原酶相关基因翻译改进

Genome-wide association study using specific-locus amplified fragment sequencing identifies new genes influencing nitrogen use efficiency in rice landraces

基于特定位点扩增片段测序的全基因组关联研究发现影响水稻地方品种氮素利用效率的新基因

Genome-wide Association Study of a Panel of Vietnamese Rice Landraces Reveals New QTLs for Tolerance to Water Deficit During the Vegetative Phase

越南水稻地方品种的大规模基因组关联研究揭示了 vegetative 生态阶段耐干旱的新 QTLs

Rapid identification of a new gene influencing low amylose content in rice landraces (Oryza sativa L.) using genome-wide association study with specific-locus amplified fragment sequencing

利用全基因组关联分析与特异位点扩增片段测序快速鉴定低支链淀粉水稻地方品种新基因

Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns

针对免疫球蛋白G糖基化模式的大规模遗传关联研究

A genome-wide association study identifies new genes associated with developmental dysplasia of the hip

全基因组关联研究发现与发育性髋关节发育不良相关的新的易感基因

Genome-wide association study of antisocial personality disorder

精神分裂症的功能表型遗传研究

Risk of lower respiratory tract infections: a genome-wide association study with Mendelian randomization analysis in three independent European populations

下呼吸道感染的风险：在三个独立的欧洲人群中进行孟德尔随机化分析的全基因组关联研究

A Genome-Wide Association Study into the Aetiology of Congenital Solitary Functioning Kidney

单侧肾脏发生的遗传背景的全基因组关联分析研究

Genome-wide association study and BSR-seq identify nitrate reductase-related genes in rice landraces (Oryza sativa L.)

基于全基因组关联分析和BSR-seq发掘水稻农家品种中的硝酸盐还原酶相关基因 翻译改进

Genome-wide association study using specific-locus amplified fragment sequencing identifies new genes influencing nitrogen use efficiency in rice landraces

基于特定位点扩增片段测序的全基因组关联研究发现影响水稻地方品种氮素利用效率的新基因

Genome-wide Association Study of a Panel of Vietnamese Rice Landraces Reveals New QTLs for Tolerance to Water Deficit During the Vegetative Phase

越南水稻地方品种的大规模基因组关联研究揭示了 vegetative 生态阶段耐干旱的新 QTLs

Rapid identification of a new gene influencing low amylose content in rice landraces (Oryza sativa L.) using genome-wide association study with specific-locus amplified fragment sequencing

利用全基因组关联分析与特异位点扩增片段测序快速鉴定低支链淀粉水稻地方品种新基因

Genome-Wide Association Study on Immunoglobulin G Glycosylation Patterns

针对免疫球蛋白G糖基化模式的大规模遗传关联研究

A genome-wide association study identifies new genes associated with developmental dysplasia of the hip

全基因组关联研究发现与发育性髋关节发育不良相关的新的易感基因

Genome-wide association study of antisocial personality disorder

精神分裂症的功能表型遗传研究

Risk of lower respiratory tract infections: a genome-wide association study with Mendelian randomization analysis in three independent European populations

下呼吸道感染的风险：在三个独立的欧洲人群中进行孟德尔随机化分析的全基因组关联研究

A Genome-Wide Association Study into the Aetiology of Congenital Solitary Functioning Kidney

单侧肾脏发生的遗传背景的全基因组关联分析研究

基于全基因组关联分析和BSR-seq发掘水稻农家品种中的硝酸盐还原酶相关基因翻译改进