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Review Current microbiology. 2025 Apr 24;82(6):260. doi: 10.1007/s00284-025-04226-w Q32.62025

Leveraging CRISPR-Cas-Enhanced Isothermal Amplification Tools for Quick Identification of Pathogens Causing Livestock Diseases

利用CRISPR-Cas增强等温扩增技术快速鉴别家畜病原菌 翻译改进

Ayan Mukherjee  1, Sukhen Samanta  2, Subhasree Das  3, Molla Zakirul Haque  3, Partha Sarathi Jana  3, Indranil Samanta  3, Indrajit Kar  3, Srinibas Das  3  4, Pramod Kumar Nanda  5, Prasad Thomas  6, Premanshu Dandapat  7

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作者单位

  • 1 Faculty of Veterinary and Animal Sciences, West Bengal University of Animal & Fishery Sciences, Mohanpur, West Bengal, 741 252, India. dramukherjee@wbuafscl.ac.in.
  • 2 Department of Microbiology, University of Kalyani, Nadia, West Bengal, 741 235, India.
  • 3 Faculty of Veterinary and Animal Sciences, West Bengal University of Animal & Fishery Sciences, Mohanpur, West Bengal, 741 252, India.
  • 4 Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata, 700 094, West Bengal, India.
  • 5 ICAR-Indian Veterinary Research Institute, Eastern Regional Station, Belgachia Road, Kolkata, West Bengal, 700 037, India.
  • 6 ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India.
  • 7 ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India. pdandapat@gmail.com.

    • DOI: 10.1007/s00284-025-04226-w PMID: 40274667

    摘要 中英对照阅读

    Prompt and accurate diagnosis of infectious pathogens of livestock origin is of utmost importance for epidemiological surveillance and effective therapeutic strategy formulation. Among various methods, nucleic acid-based detection of pathogens is the most sensitive and specific; but the majority of these assays need expensive equipment and skilled workers. Due to the rapid advancement of clustered regularly interspaced short palindromic repeats-CRISPR-asso... ...点击完成人机验证后继续浏览

    动物源性病原体的准确诊断对于流行病学监测和有效治疗策略的制定至关重要。在各种方法中，基于核酸的病原体检测是最敏感和最特异的方法；然而，大多数这些检测试验需要昂贵的设备和熟练的技术人员。由于CRISPR-Cas（成簇规律间隔短回文重复序列-相关蛋白）基序核酸检测方法的快速发展，这类技术现在被广泛用于病原体检测。CRISPR-Cas是细菌中类似于“适应性免疫”的系统，通常用于基因编辑。许多CRISPR系统已被修改以用于核酸检测，因为它们在检测DNA和RNA序列方面具有出色的特异性。将CRISPR与适当的等温扩增技术结合使用，使其更敏感、更特异、更具通用性和可重复性，可以在现场即时检测病原体核酸。通过等温扩增对病原体核酸进行扩增后采用CRISPR-Cas基序的检测方法具有多种优势，包括从样本到结果的时间短且不需要实验室设置。这些方法也比现有的核酸检测方法便宜得多。这篇综述重点关注了最近在使用这种精准诊断方法来诊断各种动物病原体（无论是否具有人畜共患病潜力）的趋势，特别是各种等温扩增策略和用于检测兽医及公共卫生重要细菌、病毒和寄生虫的可视化方法。

    © 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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    期刊名：Current microbiology

    缩写：CURR MICROBIOL

    ISSN：0343-8651

    e-ISSN：1432-0991

    IF/分区：2.6/Q3

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