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CRISPR/Cas-mediated mRNA knockdown in the embryos of Xenopus tropicalis

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The Xenopus tropicalis (Western clawed frog) is an important amphibian model for genetics, developmental and regenerative biology, due to its diploid genetic background and short generation time. CRISPR-Cas13 and CRISPR interference (CRISPRi) systems have rece... ...