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Environmental microbiology. 2025 Apr;27(4):e70082. doi: 10.1111/1462-2920.70082 Q24.32024

Needle in a Haystack: Culturing Plant-Beneficial Helotiales Lineages From Plant Roots

针在草堆:从植物根系中培养有益于植物的Helotiales谱系 翻译改进

Pauline Bruyant  1, Jeanne Doré  1, Laurent Vallon  1, Yvan Moënne-Loccoz  1  2, Juliana Almario  1

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作者单位

  • 1 Laboratoire d'Ecologie Microbienne LEM, CNRS UMR5557, INRAE UMR1418, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, Villeurbanne, France.
  • 2 Institut Universitaire de France, Paris, France.
  • DOI: 10.1111/1462-2920.70082 PMID: 40228525

    摘要 中英对照阅读

    Root-associated Helotiales fungi are increasingly recognised as beneficial fungal partners promoting plant growth under nutrient-limited conditions, particularly, in non-mycorrhizal hosts lacking the ancestral arbuscular mycorrhizal symbiosis. However, the ecology of these fungi is still cryptic as relatively few lineages have been successfully cultivated from roots for further study. Here, we attempted the mass isolation of root endophytic fungi to evaluate the recovery of known plant-beneficial Helotiales lineages using a tailored culture-based approach. We sampled six wild non-mycorrhizal species from the Brassicaceae, Caryophyllaceae, and Cyperaceae, growing in nutrient-limited alpine soils. We isolated 602 root endophytes and compared this culturable diversity with the one observed via fungal ITS2 metabarcoding. Metabarcoding revealed that Helotiales taxa dominated the fungal communities, with 43% of these detected taxa also represented in our collection. Accordingly, most root endophytes in our collection (53%) were Helotiales. These isolates, some with P solubilisation potential, belonged primarily to three Helotialean clades and were phylogenetically related to plant growth-promoting or mycorrhizal-like strains. This analysis highlights that the roots of alpine non-mycorrhizal plants harbour diverse plant-beneficial root-endophytic Helotiales, and the isolates obtained are a promising resource to explore the plant-beneficial mechanisms and ecological traits of these fungi.

    Keywords: Brassicaceae; Caryophyllaceae; Cyperaceae; Helotiales; fungal collection; non‐mycorrhizal plants; root endophytes.

    Keywords:needle in haystack; plant roots

    根相关的Helotiales真菌越来越被认为是促进植物在营养限制条件下生长的有益共生伙伴,尤其是在缺乏祖先丛枝菌根共生关系的非菌根宿主中。然而,这些真菌的生态学仍然是神秘的,因为只有少数谱系成功地从根部培养出来以供进一步研究。在这里,我们尝试大规模分离根内生真菌,以便使用定制的基于培养的方法评估已知对植物有益的Helotiales谱系的回收情况。我们采集了六种生长在营养限制性高山土壤中的野生非菌根物种(十字花科、石竹科和莎草科)。我们从这些样本中分离出了602个根内生真菌,并将其可培养多样性与通过真菌ITS2 metabarcoding观察到的多样性进行了比较。Metabarcoding显示,Helotiales类群主导了真菌群落,其中43%的检测到的分类单元也出现在我们的收藏中。相应地,在我们收集中的大多数根内生真菌(53%)属于Helotiales。这些分离株中的一些具有磷溶解潜力,并且主要属于三个Helotialean支系,并与促进植物生长或类似菌根的菌株在系统发育上相关。这一分析强调了高山非菌根植物的根部藏有多种对植物有益的根内生Helotiales,而获得的分离株是研究这些真菌的植物益处机制和生态特征的宝贵资源。

    关键词:十字花科;石竹科;莎草科;Helotiales;真菌收集;非菌根植物;根内生菌。

    关键词:针在草堆中; 植物根部

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    期刊名:Environmental microbiology

    缩写:ENVIRON MICROBIOL

    ISSN:1462-2912

    e-ISSN:1462-2920

    IF/分区:4.3/Q2

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