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Environmental microbiology. 2025 Apr;27(4):e70096. doi: 10.1111/1462-2920.70096 Q24.32024

Methanol and Carbon Monoxide Metabolism of the Thermophile Moorella caeni

莫尔拉氏菌耐温株甲醇和一氧化碳代谢 翻译改进

Nicolas A Vecchini Santaella  1, Alfons J M Stams  1, Diana Z Sousa  1

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  • 1 Laboratory of Microbiology, Wageningen University & Research, Wageningen, the Netherlands.
  • DOI: 10.1111/1462-2920.70096 PMID: 40228503

    摘要 中英对照阅读

    Moorella species are thermophilic acetogens that primarily produce acetate from one-carbon (C1) compounds including CO, CO2 (+H2), methanol and formate. Notably, Moorella caeni DSM 21394T displays a hydrogenogenic metabolism on CO and an acetogenic metabolism on methanol. Furthermore, M. caeni is unable to use CO2 (+H2) and grows only on formate in the presence of a methanogen or when thiosulfate is added as an electron acceptor. Presently, all theoretical frameworks for C1 metabolism in Moorella species are derived from experimental and genomic analyses of Moorella thermoacetica, which exhibits an acetogenic metabolism with all C1 substrates. In this study, we applied a transcriptomics approach to elucidate the mechanisms underlying the C1 metabolism of Moorella caeni during growth on methanol and CO. Our results indicate that respiratory Complex 1, a proton-translocating (ubi)quinone oxidoreductase, is the primary respiratory enzyme in methanol-grown cells of M. caeni. Conversely, in CO-grown cells, an energy-conserving hydrogenase complex (Ech) appears to be the primary respiratory complex, alongside respiratory Complex 1. This study provides insight into the C1 metabolism of M. caeni and reveals variations in gene syntenies related to C1 metabolism among the Moorella genus.

    Keywords: Ech complex; acetogenesis; acetogens; carbon monoxide; hydrogenogenesis; methanol; respiratory complex 1.

    Keywords:methanol metabolism; carbon monoxide; moorella caeni

    莫尔氏菌属物种是一类嗜热乙酸生成菌,主要通过一碳(C1)化合物包括CO、CO2 (+H2)、甲醇和甲酸来产生乙酸。值得注意的是,M. caeni DSM 21394T 在CO上表现出氢生成代谢,在甲醇上表现出乙酸生成代谢。此外,M. caeni 无法利用 CO2 (+H2) 并且仅在有产甲烷菌存在或添加硫代硫酸盐作为电子受体时才以甲酸为生长底物。目前,莫尔氏菌属物种的所有理论框架都是基于对 M. thermoacetica 的实验和基因组分析得出的,该物种对所有C1底物都表现出乙酸生成代谢。在这项研究中,我们应用转录组学方法来阐明M. caeni在生长于甲醇和CO时的一碳代谢机制。我们的结果表明,在甲醇培养条件下,呼吸复合体1,一种质子转运(泛醌)氧化还原酶是主要的呼吸酶。相反地,在一氧化碳培养细胞中,能量守恒氢化物复合体(Ech)似乎是主要的呼吸复合体,与呼吸复合体1一起作用。这项研究揭示了M. caeni的一碳代谢机制,并揭示了莫尔氏菌属物种间有关一碳代谢基因串联排列的差异。

    关键词:Ech 复合体;乙酸生成;乙酸生成菌;一氧化碳;氢生成;甲醇;呼吸复合体1.

    关键词:甲醇代谢; 一氧化碳; Moorella thermoacetica

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    期刊名:Environmental microbiology

    缩写:ENVIRON MICROBIOL

    ISSN:1462-2912

    e-ISSN:1462-2920

    IF/分区:4.3/Q2

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