Journal of medical entomology. 2025 Apr 11:tjaf044. doi: 10.1093/jme/tjaf044 Q12.02025

Emerging approaches to mosquito species identification: an overview with emphasis on nanopore sequencing technology

蚊虫种类鉴定新方法概述：以纳米孔测序技术为重点翻译改进

Antsa Rakotonirina¹, Rutaiwan Dusadeepong², Mallorie Hide^{3 4 5}, Linavin Vuth¹, Rina Chea¹, Kimly Heng¹, Pierre-Olivier Maquart^{1 6}, Koen Vandelannoote², Sébastien Boyer^{1 7}

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作者单位

¹ Medical and Veterinary Entomology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.

² Bacterial Phylogenomics Group, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.

³ Medical Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.

⁴ MIVEGEC, Montpellier University, CNRS, IRD, Montpellier, France.

⁵ LMI Drug Resistance in South East Asia, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.

⁶ UMR EGCE IRD, CNRS, Université Paris-Saclay, Gif sur Yvette Cedex, France.

⁷ Ecology & Emergence of Arthropod-borne Pathogens Unit, Department of Global Health, Institut Pasteur, CNRS UMR2000, Paris, France.

DOI: 10.1093/jme/tjaf044 PMID: 40214625

摘要中英对照阅读

Correct identification of mosquito species is crucial for effective vector control, pathogen surveillance, and research and can be achieved through various methods. In this study, we present a review of the most commonly used method for mosquito species identification. We also benchmark the results of DNA sequencing on the MinION nanopore sequencing device against the morphological identification technique, which remains the cornerstone of mosquito species... ...点击完成人机验证后继续浏览

正确识别蚊虫种类对于有效的媒介控制、病原体监测和研究至关重要，可以通过多种方法实现。在这项研究中，我们回顾了最常用的一种蚊虫种类鉴定方法，并将MinION纳米孔测序设备的DNA测序结果与形态学鉴定技术进行对比，后者仍然是蚊虫物种识别的基础。通过使用95个野外样本，我们针对两个线粒体基因（细胞色素氧化酶亚基1和3）进行了目标扩增子的Oxford Nanopore Technology测序，并成功区分了Vishnui亚群中的三种库蚊种类（Culex pseudovishnui、Cx. tritaeniorhynchus 和 Cx. vishnui），而形态学方法则效果较差。尽管基于扩增子的目标Oxford Nanopore Technology测序成本较高，但只要资金充足，它在提高蚊虫物种鉴定准确度方面显示出巨大的潜力。

关键词： Culicidae；纳米孔技术；鉴定。

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期刊名：Journal of medical entomology

缩写：J MED ENTOMOL

ISSN：0022-2585

e-ISSN：1938-2928

IF/分区：2.0/Q1

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Emerging approaches to mosquito species identification: an overview with emphasis on nanopore sequencing technology

蚊虫种类鉴定新方法概述：以纳米孔测序技术为重点翻译改进

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Emerging approaches to mosquito species identification: an overview with emphasis on nanopore sequencing technology

蚊虫种类鉴定新方法概述：以纳米孔测序技术为重点 翻译改进

The Applications of Nanopore Sequencing Technology in Pathogenic Microorganism Detection

纳米孔测序技术在病原微生物检测中的应用

Nanopore sequencing technology and its applications

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Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology

使用牛津纳米孔测序技术鉴定台湾新兴黄瓜病毒

Nanopore sequencing technology, bioinformatics and applications

纳米孔测序技术、生物信息学及应用

Nanopore Sequencing Technology in Oral Oncology: A Comprehensive Insight

纳米孔测序技术在口腔肿瘤学中的综合见解

Transcriptome annotation of 17 porcine tissues using nanopore sequencing technology

使用纳米孔测序技术对17种猪组织进行转录组注释

Applying nanopore sequencing technology in Paracoccidioides sp.: a high-quality DNA isolation method for next-generation genomic studies

纳米孔测序技术在副球孢子菌属中的应用——下一代基因组研究的高质量DNA分离方法

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO

纳米孔测序技术：快速检测非法转基因食品的新途径

Draft Genome Sequence of an Environmental Vibrio cholerae Strain, 2012Env-25, Obtained Using Nanopore Sequencing Technology

使用纳米孔测序技术获得的环境霍乱弧菌株2012Env-25的草图基因组序列

蚊虫种类鉴定新方法概述：以纳米孔测序技术为重点翻译改进