Malaysia's malaria rate has declined but remains a public health concern, with limited investigations into malaria and coinfections with soil-transmitted helminth (STH) infections. A cross-sectional study using convenience sampling in Orang Asli villages enrolled 437 villagers aged 1-83 years based on their willingness to participate. Blood samples were tested microscopically for malaria, followed by nested polymerase chain reaction (PCR), and stool samples were screened microscopically for STH eggs. Body temperature, demographic, and socioeconomic data were collected. Malaria parasite was detectable only via PCR, with a 15.3% prevalence, indicating submicroscopic malaria parasitemia; none of the positive cases presented fever. The identified species included Plasmodium vivax (8.7%), Plasmodium cynomolgi (5.5%), Plasmodium knowlesi (4.3%), Plasmodium falciparum (1.8%), Plasmodium inui (0.2%), and Plasmodium malariae (0.2%). Females had significantly higher rates of submicroscopic malaria parasitemia (19.6%) compared with males (9.3%, P = 0.003). STH infections were highly prevalent (71.4%), with Trichuris trichiura (65.2%), Ascaris lumbricoides (35.0%), and hookworm (14.6%). STH infection was associated with age (P <0.001), peaking in individuals aged 10-19 years (86.2%) and 1-9 years (83.0%), as well as with students (84.3% versus 60.8% in employed and 60.3% in unemployed; P <0.001) and low-income households (76.4% versus 61.7% in higher-income households; P = 0.002). Submicroscopic malaria parasitemia and STH coinfections were present in 8.9% of participants, with higher rates in low-income households (12.6% versus 5.2% in higher-income, P = 0.010). The Negrito tribe exhibited the highest prevalence of submicroscopic malaria parasitemia, STH, and coinfections (P <0.05). This study highlights the need for integrated malaria and STH control strategies, particularly for the Negrito tribe.

马来西亚的疟疾发病率有所下降，但仍是一个公共卫生问题，并且对疟疾与土壤传播蠕虫（STH）感染合并感染的研究有限。一项使用便利抽样法在原住民村庄进行的横断面研究招募了437名年龄在1至83岁之间的村民参与，参与者基于他们的自愿性被纳入研究。血液样本通过显微镜检测疟疾，并随后进行套式聚合酶链反应（PCR）检测；粪便样本通过显微镜筛查土壤传播蠕虫卵。收集身体温度、人口统计和经济社会数据。只有通过PCR才能检测到疟原虫，其检出率为15.3%，表明存在亚显微疟疾病原体感染；没有阳性病例表现出发热症状。鉴定出的种类包括间日疟原虫（8.7%）、猕猴疟原虫（5.5%）、凯氏疟原虫（4.3%）、恶性疟原虫（1.8%）、环形泰氏疟原虫（0.2%）和卵形疟原虫（0.2%）。女性的亚显微疟疾病原体感染率显著高于男性（分别为19.6% 和 9.3%，P = 0.003）。土壤传播蠕虫感染高度普遍，占比71.4%，其中主要是直肠线虫（65.2%）、蛔虫（35.0%）和钩虫（14.6%）。土壤传播蠕虫感染与年龄有关（P <0.001），在10至19岁年龄段（86.2%）以及1至9岁年龄段（83.0%）达到峰值，同时学生群体的感染率最高（84.3%，相比在职人员的60.8%，失业者的60.3%；P <0.001），且在低收入家庭中更为普遍（76.4%，相较高收入家庭中的61.7%；P = 0.002）。参与者中有8.9%的人同时存在亚显微疟疾病原体感染和土壤传播蠕虫合并感染，这种现象在低收入家庭中更加明显（分别为12.6% 和 高收入家庭的5.2%，P = 0.010）。尼格利陀部落表现出最高的亚显微疟疾、土壤传播蠕虫及其合并感染的发生率（P <0.05）。这项研究强调了对尼格利陀部落采取综合控制措施以应对疟疾和土壤传播蠕虫的重要性。