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Current microbiology. 2025 Apr 5;82(5):233. doi: 10.1007/s00284-025-04197-y Q32.62025

Isolating, Screening, and Optimizing Antioxidant Production of Endophytic Bacteria From Zingiber officinale Roscoe (Ginger)

来自Zingiber officinale Roscoe（生姜）内生细菌的抗氧化剂产生菌株的分离、初筛及优化研究 翻译改进

Trang Thi Xuan Dai  1, Tuan Thanh Chau  2, Linh Chi Tran  3  4, Ta Lam Tai  2

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作者单位

  • 1 Department of Biology, College of Natural Sciences, Can Tho University, Campus II, 3-2 Street, Ninh Kieu District, Can Tho City, 94000, Viet Nam. dtxtrang@ctu.edu.vn.
  • 2 Institute of Food and Biotechnology, Can Tho University, Campus II, 3-2 Street, Ninh Kieu District, Can Tho City, 94000, Viet Nam.
  • 3 Department of Biology, College of Natural Sciences, Can Tho University, Campus II, 3-2 Street, Ninh Kieu District, Can Tho City, 94000, Viet Nam.
  • 4 Faculty of Medicine, Nam Can Tho University, 168, Nguyen Van Cu (Extension Road), Ninh Kieu District, Can Tho City, 94000, Viet Nam.

    • DOI: 10.1007/s00284-025-04197-y PMID: 40186780

    摘要 中英对照阅读

    Zingiber officinale Roscoe (ginger) is widely used as a herb in traditional cuisines and medicines. This study aimed to identify endophytic bacteria with high antioxidative potential in ginger plants and determine the optimum conditions for antioxidant fermentation from the strain. The bacteria from ginger were isolated and the growth conditions were surveyed. The secondary metabolites of the endophytes extracted using ethyl acetate were used to determine ... ...点击完成人机验证后继续浏览

    zingiber officinale Roscoe（姜）在传统烹饪和医药中被广泛用作草药。本研究旨在识别姜植物内生细菌的高抗氧化潜力，并确定该菌株抗氧化发酵的最佳条件。从姜中分离出细菌并调查其生长条件。使用乙酸乙酯提取内生微生物次级代谢产物，测定总酚含量（TPC）、总黄酮含量（TFC）和抗氧化活性。采用16S rRNA基因测序方法鉴定六种菌株。Kosakonia sp. ZO-Rh4表现出最高活性，并对其发酵条件进行了优化。结果表明，从姜组织中分离出的32株内生菌表现出显著活性。ZO-Rh4、ZO-L2、ZO-S7、ZO-S8、ZO-L7和ZO-L8菌株活性最高，分别属于Kosakonia、Pantoea、Enterobacter和Klebsiella属。在最佳发酵条件下（32.58°C，71.49小时，14.45 g/L葡萄糖，pH 7.58），Kosakonia sp. ZO-Rh4产生的TPC为285.74 mg没食子酸当量/mL，TFC为144.49 mg槲皮素当量/mL。此外，通过清除2,2-二苯基-1-苦味肼自由基活性、总抗氧化能力和还原力测定的EC50值证明了抗氧化活性显著增加。这些发现突显了内生菌，特别是Kosakonia sp. ZO-Rh4作为天然抗氧化剂具有多种应用潜力。

    © 2025. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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    期刊名：Current microbiology

    缩写：CURR MICROBIOL

    ISSN：0343-8651

    e-ISSN：1432-0991

    IF/分区：2.6/Q3

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